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Broiler Glucose Oxidase Experiment
Release time:
2020-12-24
One-day-old Ross 308 broiler chickens were selected, with a total of 4 chicken houses, each housing 11,000 birds. The birds were randomly divided into a control group and a treatment group, with 2 chicken houses assigned to each group. The control group was fed a commercial diet supplemented with Changle (allicin and oregano phenol) at 100 mL/ton, while the treatment group was fed a commercial diet supplemented with glucose oxidase at 100 U/L. During the trial period, birds had free access to feed and water. The trial lasted for 42 days. The experimental diet consisted of corn, soybean meal, wheat, cottonseed meal, DDGS, peanut meal, duck fat, and premixes, among other ingredients; nutritional parameters are shown in Table 1.
The Effect of Glucose Oxidase on the Production Performance of Broilers
Test location: A chicken farm in Jinan
Test period: 2015.6.10–2015.7.22
Test subjects: Broiler chickens aged 1–42 days
Objective of the trial: To add glucose oxidase to drinking water and observe its impact on the production performance of broiler chickens.
Materials and Methods:
1. Test materials
Glucose oxidase is supplied by Jinan Tiantianxiang Co., Ltd., and Changle is supplied by Heilongjiang Huifeng Animal Health Products Co., Ltd.
2. Experimental Animals and Experimental Design
One-day-old Ross 308 broiler chickens were selected, with a total of 4 chicken houses, each housing 11,000 birds. The birds were randomly divided into a control group and a treatment group, with 2 chicken houses assigned to each group. The control group was fed a commercial diet supplemented with Changle (allicin and oregano phenol) at 100 mL/ton, while the treatment group was fed a commercial diet supplemented with glucose oxidase at 100 U/L. During the trial period, birds had free access to feed and water. The trial lasted for 42 days. The experimental diet consisted of corn, soybean meal, wheat, cottonseed meal, DDGS, peanut meal, duck fat, and premixes, with nutritional composition shown in Table 1.
Table 1. Dietary Nutritional Levels
| 1-21 days old |
22 - To be marketed |
||
| Nutritional Indicator (Aiyinong 510) |
Nutritional indicators (He Mei 511) |
||
| Crude protein, % |
21 |
Crude protein, % |
19 |
| Crude fiber, % |
6 |
Crude fiber, % |
7 |
| Crude ash, % |
8 |
Crude ash, % |
8 |
| Crude fat, % |
3 |
Crude fat, % |
3 |
| Calcium, % |
0.8–1.3 |
Calcium, % |
0.7–1.2 |
| Total phosphorus, % |
0.5 |
Total phosphorus, % |
0.55 |
| Lysine, % |
1 |
Lysine, % |
0.9 |
| Methionine, % |
0.5 |
Methionine, % |
0.35–0.9 |
| Sodium chloride, % |
0.3–0.8 |
Sodium chloride, % |
0.3–0.8 |
| Moisture, % |
14 |
Moisture, % |
14 |
3. Test items
Record the feed consumption daily, weigh the animals at 21 days and at the time of slaughter, and measure feed consumption in each pen during the trial period. Calculate the average daily feed intake and average daily weight gain for each phase, and determine the feed-to-meat ratio. During the trial, observe the feces, incidence of diarrhea, and overall condition of the flock.
Test results:
1. The Effect of Glucose Oxidase on the Production Performance of Broilers
Table 2. Effect of Glucose Oxidase on the Production Performance of Broilers

As shown by the results, the addition of glucose oxidase had a positive effect on the overall condition of the chicken flock. In terms of feed-to-meat ratio, from 1 to 21 days of age, the experimental group showed a 2.95% reduction compared to the control group; from 22 days of age until market time, the reduction was 7.50%; and over the entire rearing period, the feed-to-meat ratio was reduced by 5.99%. Regarding average daily weight gain, the addition of glucose oxidase significantly improved the daily weight gain of chickens in the experimental group, with particularly noticeable improvements in the later stage (from 22 days of age until market time), resulting in chickens at 42 days of age being significantly heavier than those in the control group. There was no significant difference in average feed consumption between the control and experimental groups.
2. The Effect of Glucose Oxidase on Broiler Manure
After adding glucose oxidase (at 5 days of age), a difference in the incidence of diarrhea between the experimental group and the control group became apparent. The control group chickens showed a greater number of cases of fecal soiling around the cloaca, whereas the experimental group exhibited milder symptoms, as shown in Figure 1.
Figure 1. Effect of glucose oxidase on broiler manure

3. Results
The experimental results show that the addition of glucose oxidase reduced the feed-to-meat ratio and improved the production performance of broilers. In this experiment, the treatment group using glucose oxidase demonstrated certain advantages over the control group using Changle. The underlying reason may be that glucose oxidase acts on glucose in the feed, producing gluconic acid and thereby lowering the pH of the intestinal tract. This creates a favorable pH environment that enables digestive enzymes to function more effectively, increases the activation rate of proenzymes, enhances the activity of digestive enzymes, and ultimately improves the digestibility and absorption of nutrients.
Meanwhile, the reaction consumes oxygen, creating an anaerobic environment that improves the gut microbiota, promotes the growth and reproduction of beneficial bacteria such as lactobacilli and bifidobacteria, and inhibits the growth of harmful bacteria like Escherichia coli and Salmonella.
Additionally, reports indicate that the addition of glucose oxidase promotes the development of the broiler’s intestinal tract, significantly optimizing the morphological structure of the broiler’s small intestine and providing a favorable material foundation for the maturation of the digestive tract and efficient digestion and absorption.
As can be seen, the addition of glucose oxidase provides broilers with a better gastrointestinal digestive environment, thereby improving the overall physiological condition of the broilers and resulting in enhanced production performance.
Note: The above experimental data are obtained from experiments conducted independently by the Tiantianxiang Laboratory.
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One-day-old Ross 308 broiler chickens were selected, with a total of 4 chicken houses, each housing 11,000 birds. The birds were randomly divided into a control group and a treatment group, with 2 chicken houses assigned to each group. The control group was fed a commercial diet supplemented with Changle (allicin and oregano phenol) at 100 mL/ton, while the treatment group was fed a commercial diet supplemented with glucose oxidase at 100 U/L. During the trial period, birds had free access to feed and water. The trial lasted for 42 days. The experimental diet consisted of corn, soybean meal, wheat, cottonseed meal, DDGS, peanut meal, duck fat, and premixes, among other ingredients; nutritional parameters are shown in Table 1.
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